A spectrophotometric assay for feruloyl esterases.
نویسندگان
چکیده
We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.
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Feruloyl esterase
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ورودعنوان ژورنال:
- Analytical biochemistry
دوره 309 1 شماره
صفحات -
تاریخ انتشار 2002